でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。
WARP
Target | Primer name | Sense primer (5'->3') | Anti-sense primer (5'->3') | Product size [bp] | Annealing temperature[oC] |
---|---|---|---|---|---|
Exon1 | WARP_E1F/E1R | TGCGTGCCTCGGTCGTATC | GCAGAGCGGTCACCAGAAG | 386 | * |
Exon2 | WARP_E2-1F/E2-1R | TGGGAGAAGGCAAGCATCCAC | CTGTTCCTTGGCATAGACCAGC | 410 | * |
Exon2 | WARP_E2-2F/E2-2R2 | CAGGATGCGGTGCGTGCTTC | TGCATAGACAAGCTGCATCACTCAG | 505 | * |
Exon3 | WARP_E3F/E3R | GCCTCCAATCTCCAGATCTTCC | GAGATGACGATGCGCTCTGG | 488 | * |
Exon4 | WARP_E4F/E4SR3 | CTAGTGCCTGAGTCCAACGTG | TGCACCCGTCTGCCGGGTTG | 589 | * |
Exon4 | WARP_E4SF/E4SR4 | TCGGCTACCACGTGCAGTTC | GAGTCCGCGTGGGGAAAG | 377 | * |
*Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 95 oC for 30 sec annealing at 60 oC for 1 min extension at 72 oC for 1 min × 5 Cycles, and denaturatiion at 95 oC for 30 sec annealing at 55 oC for 1 min extention at 72 oC for 1 min × 35 Cycles
Target | Primer name | Sequence |
---|---|---|
Exon1 | WARP_E1R | GCAGAGCGGTCACCAGAAG |
Exon2 | WARP_E2-1R | CTGTTCCTTGGCATAGACCAGC |
Exon2 | WARP_E2-2R | TGCATAGACAAGCTGCATCACTCAG |
Exon3 | WARP_E3F | GCCTCCAATCTCCAGATCTTCC |
Exon4 | WARP_4F | CTAGTGCCTGAGTCCAACGTG |
Exon4 | WARP_4SF | TCGGCTACCACGTGCAGTTC |
連絡先
お問い合わせは、
山田(taka0197@med.hokudai.ac.jp)宛