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TRPV4

TRPV4 PCR Primer
Target	Primer name	Sequence	Product size [bp]	Annealing temperature [oC]	DMSO	GC Buffer (LA Taq)
Exon 1	TRPV4-5UTR-F	AGACCGTCTGCCGCTCTATC	460	60		I
Exon 1	TRPV4-5UTR-R	AACCTGGTGCCTTCCGTCT	460	60		I
Exon 2	TRPV4-ex1F	AGCACTGCTGCTTGTCCA	580	60	-
Exon 2	TRPV4-ex1R	AGAGGGGTGGATGATGAATG	580	60	-
Exon 3	TRPV4-ex2F	GGGCAGTCTGTGCATTTCTT	499	60	-
Exon 3	TRPV4-ex2R	AAAGAAGCTGAGGGGAAAGG	499	60	-
Exon 4	TRPV4-ex3F	CCCATGCGTTTATTGTACCC	462	60	-
Exon 4	TRPV4-ex3R	GCACTTAGGCCCAGATCAAA	462	60	-
Exon 5	TRPV4-ex4F	AGGACCTATTTGCTGCCCTT	505	60	-
Exon 5	TRPV4-ex4R	TACTCCCCATGGCACTTACA	505	60	-
Exon 6	TRPV4-ex5F	CCAGAGAAACGTGCAGTTCA	596	60	-
Exon 6	TRPV4-ex5R	TTCTTGAGCTGGGACATCTG	596	60	-
Exon 7	TRPV4-ex6F	TGCTTAGCTGTTTTCTGGGG	431	60	-
Exon 7	TRPV4-ex6R	GTTGGCACCTTCTCTTGCAT	431	60	-
Exon 8	TRPV4-ex7F	GCTGAGGAATGGATGGAAAG	374	60	O
Exon 8	TRPV4-ex7R_2	GGACCCAGAAGGATGAGGTT	374	60	O
Exon 9	TRPV4-ex8F	CATGGGATGCCGATGTTT	449	60	-
Exon 9	TRPV4-ex8R	AAAGGGATAGAAGAGAGGGAGG	449	60	-
Exon 10	TRPV4-ex9F	TTCACCAACGTAAGTGCCTG	486	60	-
Exon 10	TRPV4-ex9R	TGAATCTGGACGACCTAGCA	486	60	-
Exon 11	TRPV4-ex10F	AGAAGGCATTGGGAAGTCCT	452	60	-
Exon 11	TRPV4-ex10R	ACTTTCCCCATTCCTCCATC	452	60	-
Exon 12	TRPV4-ex11F	GCTGGATGAATGCCCTTTAC	425	60	-
Exon 12	TRPV4-ex11R	ATCATGGCTACTGTTCCCGT	425	60	-
Exon 13	TRPV4-ex12F	GCTTTCTCCTCCAGCAAATG	553	60	-
Exon 13	TRPV4-ex12R	GATGCCCATTTTACCGACAG	553	60	-
Exon 14	TRPV4-ex13F	TGTCTCATTTCTGCCACCAG	301	60	-
Exon 14	TRPV4-ex13R	ATTGCAATGGGTAGACGACG	301	60	-
Exon 15	TRPV4-ex14F	GCATGGAGAGCGTCTCTGA	325	60	-
Exon 15	TRPV4-ex14R	CATTCACAAGCAGCAGAGGA	325	60	-
Exon 16	TRPV4-ex15F	TCCCTGTGTGGAGAAAACCT	511	60	-
Exon 16	TRPV4-ex15R	ATGCGGCTGGACTAGAAATG	511	60	-
3 UTR	TRPV4-3UTR-F	CGCAAGTGGAGGACTGATGA	631	60		I
3 UTR	TRPV4-3UTR-R	ATCTCCCTCGCTGCAGTCTC	631	60		I

管理・運営：骨系統疾患コンソーシウム

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山田（taka0197@med.hokudai.ac.jp）宛

でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。