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MATN3

MATN3 PCR Primer
TargetPrimer nameSense primer (5'->3')Anti-sense primer (5'->3')Product size [bp]Annealing temperature[oC]
Exon1MATN3_1F2_ito/1R2_itoAAGGCTCCTTGTTGGCCTTGTCTCCTCGTTCGATGCTCAC424*1
Exon2MATN3_ex2/F / ex2/R981*2
Exon3MATN3_3F_ito/3R_itoTCTATTGACATGGGAGACGGCTGGAGCATAGGTTCCCAAG258*3
Exon4MATN3_4F_ito/4R_itoTCTGCCATGCTCTGCATGCTTCCCAGTCAATGTGACCTG263*3
Exon5MATN3_5F_ito/5R_itoTTCCTCATATGTTTCCAGCTGCTTTGCTGGTGAGTTGCAAG297*3
Exon6MATN3_6F_ito/6R_itoTGACTACTACATTCTCAGCACGAGAAAGAATCACAACTGTGTC342*3
Exon7MATN3_7F_ito/7R_itoCTTAGGTAAGTCTTAACGGTGGGCTCGATCGTAGTACCTTG283*3
Exon8MATN3_8F_ito/8R_itoGACCACTGCAGGGGTCTACATTAAGTACACCAAGCTGTCC315*3

*1 Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 94 oC for 30 sec annealing at 62 oC for 1 min extension at 72 oC for 1 min × 5 Cycles, denaturation at 94 oC for 1 min annealing at 60 oC for 1 min extension at 72 oC for 1 min × 10 Cycles, and denaturatiion at 94 oC for 30 sec annealing at 58 oC for 1 min extention at 72 oC for 1 min × 25 Cycles.

*2 Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 94 oC for 30 sec annealing at 60 oC for 1 min extension at 72 oC for 1 min × 35 Cycles.

*3 Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 94 oC for 30 sec annealing at 60 oC for 1 min extension at 72 oC for 1 min × 5 Cycles, denaturation at 94 oC for 1 min annealing at 55 oC for 1 min extension at 72 oC for 1 min × 10 Cycles, and denaturatiion at 94 oC for 30 sec annealing at 50 oC for 1 min extention at 72 oC for 1 min × 25 Cycles.

MATN3 Sequence Primer
TargetPrimer nameSequence
Exon1 Matn3_1R2_itoTCTCCTCGTTCGATGCTCAC
Exon2 Matn3_ex2/F
Exon2 Matn3_ex2/R
Exon3 Matn3_3R_itoCTGGAGCATAGGTTCCCAAG
Exon4 Matn3_4R_itoTTCCCAGTCAATGTGACCTG
Exon5 Matn3_5F_ito TTCCTCATATGTTTCCAGCTG
Exon6 Matn3_6R_itoGAGAAAGAATCACAACTGTGTC
Exon7 Matn3_7F_ito CTTAGGTAAGTCTTAACGGTG
Exon8 Matn3_8F_ito GACCACTGCAGGGGTCTAC
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連絡先

お問い合わせは、
遺伝子解析、DNA診断に関するもの:
山田(taka0197@med.hokudai.ac.jp)宛

診断/X線診断に関するもの:
西村 (gen-n@pc4.so-net.ne.jp)宛
治療に関するもの:
池川(sikegawa@ims.u-tokyo.ac.jp)宛

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