でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。
MATN3
| Target | Primer name | Sense primer (5'->3') | Anti-sense primer (5'->3') | Product size [bp] | Annealing temperature[oC] |
|---|---|---|---|---|---|
| Exon1 | MATN3_1F2_ito/1R2_ito | AAGGCTCCTTGTTGGCCTTG | TCTCCTCGTTCGATGCTCAC | 424 | *1 |
| Exon2 | MATN3_ex2/F / ex2/R | 981 | *2 | ||
| Exon3 | MATN3_3F_ito/3R_ito | TCTATTGACATGGGAGACGG | CTGGAGCATAGGTTCCCAAG | 258 | *3 |
| Exon4 | MATN3_4F_ito/4R_ito | TCTGCCATGCTCTGCATGC | TTCCCAGTCAATGTGACCTG | 263 | *3 |
| Exon5 | MATN3_5F_ito/5R_ito | TTCCTCATATGTTTCCAGCTG | CTTTGCTGGTGAGTTGCAAG | 297 | *3 |
| Exon6 | MATN3_6F_ito/6R_ito | TGACTACTACATTCTCAGCAC | GAGAAAGAATCACAACTGTGTC | 342 | *3 |
| Exon7 | MATN3_7F_ito/7R_ito | CTTAGGTAAGTCTTAACGGTG | GGCTCGATCGTAGTACCTTG | 283 | *3 |
| Exon8 | MATN3_8F_ito/8R_ito | GACCACTGCAGGGGTCTAC | ATTAAGTACACCAAGCTGTCC | 315 | *3 |
*1 Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 94 oC for 30 sec annealing at 62 oC for 1 min extension at 72 oC for 1 min × 5 Cycles, denaturation at 94 oC for 1 min annealing at 60 oC for 1 min extension at 72 oC for 1 min × 10 Cycles, and denaturatiion at 94 oC for 30 sec annealing at 58 oC for 1 min extention at 72 oC for 1 min × 25 Cycles.
*2 Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 94 oC for 30 sec annealing at 60 oC for 1 min extension at 72 oC for 1 min × 35 Cycles.
*3 Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 94 oC for 30 sec annealing at 60 oC for 1 min extension at 72 oC for 1 min × 5 Cycles, denaturation at 94 oC for 1 min annealing at 55 oC for 1 min extension at 72 oC for 1 min × 10 Cycles, and denaturatiion at 94 oC for 30 sec annealing at 50 oC for 1 min extention at 72 oC for 1 min × 25 Cycles.
| Target | Primer name | Sequence |
|---|---|---|
| Exon1 | Matn3_1R2_ito | TCTCCTCGTTCGATGCTCAC |
| Exon2 | Matn3_ex2/F | |
| Exon2 | Matn3_ex2/R | |
| Exon3 | Matn3_3R_ito | CTGGAGCATAGGTTCCCAAG |
| Exon4 | Matn3_4R_ito | TTCCCAGTCAATGTGACCTG |
| Exon5 | Matn3_5F_ito | TTCCTCATATGTTTCCAGCTG |
| Exon6 | Matn3_6R_ito | GAGAAAGAATCACAACTGTGTC |
| Exon7 | Matn3_7F_ito | CTTAGGTAAGTCTTAACGGTG |
| Exon8 | Matn3_8F_ito | GACCACTGCAGGGGTCTAC |
連絡先
お問い合わせは、
山田（taka0197@med.hokudai.ac.jp）宛