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MATN1
| Target | Primer name | Sense primer (5'->3') | Anti-sense primer (5'->3') | Product size [bp] | Annealing temperature[oC] |
|---|---|---|---|---|---|
| Exon2 | MATN1_E2F/E2R | TGGATCTAAGAAGCCGCACT | CAAAGTGTCTGCAGGCAAAG | 518 | |
| Exon3 | MATN1_E3F/E3R | TCAGCCTCCTCTCCTGGAC | CCCGGCACTGACATTCTATC | 479 |
*Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 94 oC for 30 sec annealing at 60 oC for 1 min extension at 72 oC for 1 min × 5 Cycles, denaturation at 94 oC for 1 min annealing at 55 oC for 1 min extension at 72 oC for 1 min × 10 Cycles, and denaturatiion at 94 oC for 30 sec annealing at 50 oC for 1 min extention at 72 oC for 1 min × 25 Cycles.
| Target | Primer name | Sequence |
|---|---|---|
| Exon2 | MATN1 E2SF | CCTGACCTGTGTCTCTGCT |
| Exon3 | MATN1 E3F | TCAGCCTCCTCTCCTGGAC |
連絡先
お問い合わせは、
山田（taka0197@med.hokudai.ac.jp）宛