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FBN2

FBN2 PCR Primer
TargetPrimer nameSense primer (5'->3')Anti-sense primer (5'->3')Product size [bp]Annealing temperature[oC]
Exon 1ex01gcgcgcagattttgtcttagaaagccgccaaactgag39660
Exon 2 ex02ggggcattgcttacgaagacaatggctgagtgcaagaggt24260
Exon 3 ex03cccaagtctgcaatgggtaatccctggcacatagaaggac35060
Exon 4 ex04gcccatgtgtgcacttataaagagaacatcactgctctaaagcacaa47860
Exon 5 ex05tattggcagcatcactgcacgcccaaactgagttcactgg 38560
Exon 6 ex06gcatttggaggcactggtatgaggccactcttggaaatca 39360
Exon 7 ex07aaccccactctcttgagaaaaaccccactaaggctacaatacacaa42660
Exon 8 ex08caaccatgaatgctctcatactcttcatacctgtttgggctgtt29660
Exon 9 ex09cctggctcaatgaatgtctgtctcccagagagcaaaatacag40060
Exon 10 ex10tttcttgctactgccagcat gtgcaagtgtgttcatacaacc50060
Exon 11 ex11agttgcaaccatgtgtctcagtaggttaagggggcacactg48565
Exon 12 ex12ccactgactggccttctctc acttttaacctcaacagccttg29660
Exon 13 ex13tgcttgcacctacctatcca cgtggtgtgtgagctttcat38960
Exon 14 ex14tgggggttgtttatggaagacacatggggaagctgaagtaa29560
Exon 15 ex15tggctgcaagtaaaccctgtggtgttttgtgactggcttct36560
Exon 16 ex16gcccttgaaaagcttccagtcaggattggatttctcttcttcag39660
Exon 17 ex17cttcctaatggtagttttggagcaatgactgactgaaatcagga41660
Exon 18 ex18tgaatggtctttactagacctgcatccaccaagaagtctgttactgtg39960
Exon 19 ex19ttaaacattgctgcccactgctgagtggaacaaaattgatgg39960
Exon 20 ex20cagacattctggcattgcattttatccgtagtaaggcctgaga28860
Exon 21 EX21gggctcaaatgatccttccttgcgtagtgaaagaggtgtcctag38260
Exon 22 ex22taagaccctgccttgcattt aaagaggagaaacgggaaactc36760
Exon 23 ex23ggaagtaggaccacacctgctggttttggactagccactgc27760
Exon 24 ex24atcttgcccaccagtacaccttgggacagaacattggtca 49960
Exon 25 ex25ggatgcattactgttgtccatcatggcagtcctttcaaacagag30060
Exon 26 ex26acttttcaagcatgcagcacgcccagagataagcaaaggtc30065
Exon 27 ex27tttctcccacgctgtatgtg tgtgctcctcaaacaacagg29060
Exon 28 ex28gctctggtgaagcacctttctcagcgccaaaagttttcc27260
Exon 29 ex29tggaatgctgcctaaaatgatggttccacttggtaatatctgg37760
Exon 30 ex30tggctccagatattaccaagtgcacgcttgtgtgtgcatgt30060
Exon 31 ex31acacgatgatttgcggatg cacagtctggtggcaggtaa37360
Exon 32 ex32ttctccatacggttgcatcttggacacccttaggcaggaat38760
Exon 33 ex33cctgcaaactaaaatgcctgtacatagtcacctgcaagtcaag38260
Exon 34 ex34gagcattaagtcaagccagtaaaacagcccttgttgtggtttcat30060
Exon 35 ex35atctgagggggcagaaaaatagctaagcagaaatctcaggaa31160
Exon 36 ex36ccaaattctttggaacagcaggcaaacactcatcacggttc28560
Exon 37 ex37gctgaaaagcaggagattgccactcccagctcaggaataaa40060
Exon 38 ex38catttgctgaatgcttgctc gctgctctgccctaggtttt23560
Exon 39 ex39tgccatctaagtgtgcgttc gaatgtgaggatgggccttt34860
Exon 40 ex40cacctatgctttgttcagtcatcgcagcttgcaagactctgaa35160
Exon 41 ex41ctacagctgtgtgggcttcatgctcttgggaatttttgga35260
Exon 42 ex42tcctagcagaagctggcattcttggttagagtctgtaagcctgtt39660
Exon 43 ex43tcccagggtacatgtagcattatcattcacgtcacactttgatga34560
Exon 44 ex44tgatcatcaaagtgtgacgtgtggggaaatccattgaagag39560
Exon 45 ex45tcctgctcattttgattcagccatggcacttgatggaactg35260
Exon 46 ex46ccgtatctcttactcattttcctcaggccaatactatggtttcagtt44760
Exon 47 ex47gtgccatttttctctgttgatgaaggagggaggcacatatca34660
Exon 48 ex48gatcagaggacccgagttgattgcagtggttggcacttag32360
Exon 49 ex49agctggctagtggacagtgaccagcatgatggtttacaattc28660
Exon 50 ex50tctgcatcccgtgttctaca catggttaaacccactctgg31660
Exon 51 ex51ggtggccctcactaaatatgctgccagtactgtgtatgtctattca49960
Exon 52 ex52gctgggatttataagcatgagccacccagggtacctgagaga29560
Exon 53 ex53gggtcttcctgggcattctaccgtatgctcaccagcag29860
Exon 54 ex54ctttgtgtatgaccgccaagggaaatctactagcaaaatgctgtc36060
Exon 55 ex55tcagaagaccacacctgtgactgatgtgggagtagtgccatt28960
Exon 56 ex56gaacccaccaagaaggatgatgtggagctcttctgtgatga38660
Exon 57 ex57gccctgcttaatttgaaagcccccttaggtctgatacgaaga51160
Exon 58 ex58catccatgttgctgttcacc ctgataatgagtgacttgcagga36860
Exon 59 ex59gaagtgagaatatggaaggaggagcacatggcataattcctga29360
Exon 60 ex60tgtggcttcacaagaaaggaggttactgaacataaataccaggac29760
Exon 61 ex61gctttttacttcccatgaaacgtttccctctgtccccaaagt 38260
Exon 62 ex62aggtttcgtttggagtgcat ctcaaccctcacaatttgttca27860
Exon 63 ex63gctttgcaaatcagtcgtcagcggtttggcttttagacac48760
Exon 64 ex64tttgctctgttggtttcgtg aaacgacgtgaactgcactg 35260
Exon 65 ex65ccctgcagctgatctctttc CAGGGAGGAAAGAAACAAGAGTT57560
FBN2 Sequence Primer
TargetPrimer nameSequence
Exon 1 ex01fgcgcgcagattttgtctt
Exon 1 ex01ragaaagccgccaaactgag
Exon 2 ex02fggggcattgcttacgaagac
Exon 2 ex02raatggctgagtgcaagaggt
Exon 3 ex03fcccaagtctgcaatgggtaa
Exon 3 ex03rtccctggcacatagaaggac
Exon 4 ex04fgcccatgtgtgcacttataaaga
Exon 4 ex04rgaacatcactgctctaaagcacaa
Exon 5 ex05ftattggcagcatcactgcac
Exon 5 ex05rgcccaaactgagttcactgg
Exon 6 ex06fgcatttggaggcactggtat
Exon 6 ex06rgaggccactcttggaaatca
Exon 7 ex07faaccccactctcttgagaaaaac
Exon 7 ex07rcccactaaggctacaatacacaa
Exon 8 ex08fcaaccatgaatgctctcatactc
Exon 9 ex09fcctggctcaatgaatgtctg
Exon 9 ex09rtctcccagagagcaaaatacag
Exon 10 ex10ftttcttgctactgccagcat
Exon 10 ex10rgtgcaagtgtgttcatacaacc
Exon 11 ex11fagttgcaaccatgtgtctcag
Exon 11 ex11rtaggttaagggggcacactg
Exon 12 ex12fccactgactggccttctctc
Exon 12 ex12racttttaacctcaacagccttg
Exon 13 ex13ftgcttgcacctacctatcca
Exon 13 ex13rcgtggtgtgtgagctttcat
Exon 14 ex14ftgggggttgtttatggaaga
Exon 14 ex14fcacatggggaagctgaagtaa
Exon 15 ex15ftggctgcaagtaaaccctgt
Exon 15 ex15rggtgttttgtgactggcttct
Exon 16 ex16fgcccttgaaaagcttccagt
Exon 16 ex16rcaggattggatttctcttcttcag
Exon 17 ex17fcttcctaatggtagttttggag
Exon 17 ex17rcaatgactgactgaaatcagga
Exon 18 ex18ftgaatggtctttactagacctgcat
Exon 18 ex18rccaccaagaagtctgttactgtg
Exon 19 ex19fttaaacattgctgcccactg
Exon 19 ex19rctgagtggaacaaaattgatgg
Exon 20 ex20fcagacattctggcattgcat
Exon 20 ex20rtttatccgtagtaaggcctgaga
Exon 21 EX21F_d_FBN2gggctcaaatgatccttcct
Exon 21 EX21R_b_FBN2tgcgtagtgaaagaggtgtcctag
Exon 22 ex22ftaagaccctgccttgcattt
Exon 22 ex22raaagaggagaaacgggaaactc
Exon 23 ex23fggaagtaggaccacacctgct
Exon 23 ex23rggttttggactagccactgc
Exon 24 ex24fatcttgcccaccagtacacc
Exon 24 ex24rttgggacagaacattggtca
Exon 25 ex25fggatgcattactgttgtccatcat
Exon 25 ex25rggcagtcctttcaaacagag
Exon 26 ex26facttttcaagcatgcagcac
Exon 26 ex26rgcccagagataagcaaaggtc
Exon 27 ex27ftttctcccacgctgtatgtg
Exon 27 ex27rtgtgctcctcaaacaacagg
Exon 28 ex28fgctctggtgaagcacctttc
Exon 28 ex28rtcagcgccaaaagttttcc
Exon 29 ex29ftggaatgctgcctaaaatga
Exon 29 ex29rtggttccacttggtaatatctgg
Exon 30 ex30ftggctccagatattaccaagtg
Exon 30 ex30rcacgcttgtgtgtgcatgt
Exon 31 ex31facacgatgatttgcggatg
Exon 31 ex31rcacagtctggtggcaggtaa
Exon 32 ex32fttctccatacggttgcatctt
Exon 32 ex32rggacacccttaggcaggaat
Exon 33 ex33fcctgcaaactaaaatgcctgt
Exon 33 ex33racatagtcacctgcaagtcaag
Exon 34 ex34fgagcattaagtcaagccagtaaaac
Exon 34 ex34ragcccttgttgtggtttcat
Exon 35 ex35fatctgagggggcagaaaaat
Exon 35 ex35ragctaagcagaaatctcaggaa
Exon 36 ex36fccaaattctttggaacagcag
Exon 36 ex36rgcaaacactcatcacggttc
Exon 37 ex37fgctgaaaagcaggagattgc
Exon 37 ex37rcactcccagctcaggaataaa
Exon 38 ex38fcatttgctgaatgcttgctc
Exon 38 ex38rgctgctctgccctaggtttt
Exon 39 ex39ftgccatctaagtgtgcgttc
Exon 39 ex39rgaatgtgaggatgggccttt
Exon 40 ex40fcacctatgctttgttcagtcatc
Exon 40 ex40rgcagcttgcaagactctgaa
Exon 41 ex41fctacagctgtgtgggcttca
Exon 41 ex41rtgctcttgggaatttttgga
Exon 42 ex42ftcctagcagaagctggcatt
Exon 42 ex42rcttggttagagtctgtaagcctgtt
Exon 43 ex43ftcccagggtacatgtagcattat
Exon 43 ex43rcattcacgtcacactttgatga
Exon 44 ex44ftgatcatcaaagtgtgacgtg
Exon 44 ex44rtggggaaatccattgaagag
Exon 45 ex45ftcctgctcattttgattcagc
Exon 45 ex45rcatggcacttgatggaactg
Exon 46 ex46fccgtatctcttactcattttcctca
Exon 46 ex46rggccaatactatggtttcagtt
Exon 47 ex47fgtgccatttttctctgttgatg
Exon 47 ex47raaggagggaggcacatatca
Exon 48 ex48fgatcagaggacccgagttga
Exon 48 ex48rttgcagtggttggcacttag
Exon 49 ex49fagctggctagtggacagtga
Exon 49 ex49rccagcatgatggtttacaattc
Exon 50 ex50ftctgcatcccgtgttctaca
Exon 50 ex50rcatggttaaacccactctgg
Exon 51 ex51fggtggccctcactaaatatgc
Exon 51 ex51rtgccagtactgtgtatgtctattca
Exon 52 ex52fgctgggatttataagcatgagc
Exon 52 ex52rcacccagggtacctgagaga
Exon 53 ex53fgggtcttcctgggcattct
Exon 53 ex53raccgtatgctcaccagcag
Exon 54 ex54fctttgtgtatgaccgccaag
Exon 54 ex54rggaaatctactagcaaaatgctgtc
Exon 55 ex55ftcagaagaccacacctgtgac
Exon 55 ex55rtgatgtgggagtagtgccatt
Exon 56 ex56fgaacccaccaagaaggatga
Exon 56 ex56rtgtggagctcttctgtgatga
Exon 57 ex57fgccctgcttaatttgaaagc
Exon 57 ex57rccccttaggtctgatacgaaga
Exon 58 ex58fcatccatgttgctgttcacc
Exon 58 ex58rctgataatgagtgacttgcagga
Exon 59 ex59fgaagtgagaatatggaaggagga
Exon 59 ex59rgcacatggcataattcctga
Exon 60 ex60ftgtggcttcacaagaaagga
Exon 60 ex60rggttactgaacataaataccaggac
Exon 61 ex61fgctttttacttcccatgaaacg
Exon 61 ex61rtttccctctgtccccaaagt
Exon 62 ex62faggtttcgtttggagtgcat
Exon 62 ex62rctcaaccctcacaatttgttca
Exon 63 ex63fgctttgcaaatcagtcgtca
Exon 63 ex63rgcggtttggcttttagacac
Exon 64 ex64ftttgctctgttggtttcgtg
Exon 64 ex64raaacgacgtgaactgcactg
Exon 65 ex65fccctgcagctgatctctttc
Exon 65 ex65rCAGGGAGGAAAGAAACAAGAGTT
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