でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。
CSS3
| Target | Primer name | Sequence | Product size [bp] | Annealing temperature [oC] | DMSO | GC Buffer (LA Taq) | カラム複製 | |
|---|---|---|---|---|---|---|---|---|
| Exon 1 | CSS3-ex1F-1 | GTGTGAGCCGGGGAAAC | 328 | ○ | 60 | + | ||
| Exon 1 | CSS3-ex1R-1 | GTGGCCCCTGGAGATCCT | ||||||
| Exon 1 | CSS3-ex1F-4 | GGATCACCAGTTTTCGAAGC | 250 | ○ | 60 | / | Ⅰ | ○ |
| Exon 1 | CSS3-ex1R-2 | ACCCCCACGTACAGGAAGTC | ||||||
| Exon 1 | CSS3-ex1F5 | TGAGCGAGAGGAAGAGACG | 257 | ○ | 60 | + | ||
| Exon 1 | CSS3-ex1-R5 | CCTCAGGCTCGCGTCCTC | ||||||
| Exon 1 | CSS3-ex1F-3 | AGTAGCCACAACGGCAGC | 419 | ○ | 60 | / | Ⅰ | ○ |
| Exon 1 | CSS3-ex1R-3 | GGCTAGGACCGGTTACAGAG | ||||||
| Exon 2 | CSS3-ex2F | TGAAAGTTCTGTCCCTTACCTTG | 448 | ○ | 60 | + | ||
| Exon 2 | CSS3-ex2R | AATGAATAGAATCGCTGCCC | ||||||
| Exon 3 | CSS3-ex3F-1 | TGCTTTAGTTTATCGCTGGTTTT | 490 | ○ | 60 | + | ||
| Exon 3 | CSS3-ex3R-1 | GGACGGTATCATCCAGTGCT | ||||||
| Exon 3 | CSS3-ex3F-2 | CTCGGGAGAGAAATGAAGTGA | 520 | ○ | 60 | + | ||
| Exon 3 | CSS3-ex3R-2 | CTTCCGATGAGAGGAACGAG | ||||||
| Exon 3 | CSS3-ex3F-3 | AAGAAATGGGAGGGCACAAT | 520 | ○ | 60 | + | ||
| Exon 3 | CSS3-ex3R-3 | CAGGTGATGCCATATCCATAG | ||||||
| Exon 3 | CSS3-ex3F-4 | GGAAATCCTCCCACTGATGA | 519 | ○ | 60 | - | ||
| Exon 3 | CSS3-ex3R-4 | CCAAGAGTAGTTTAAGACCAGGACA |
連絡先
お問い合わせは、
山田（taka0197@kuhp.kyoto-u.ac.jp）宛