でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。
CSS2
| Target | Primer name | Sequence | Product size [bp] | Annealing temperature [oC] | DMSO | GC Buffer (LA Taq) | カラム複製 | |
|---|---|---|---|---|---|---|---|---|
| Exon 1 | CSS2-ex1F-1 | AGCTGCTGGAGGGGAATC | 270 | ○ | 60 | / | Ⅰ | ○ |
| Exon 1 | CSS2-ex1R-1 | GGTGAAGCCCAGGGAGAT | ||||||
| Exon 1 | CSS2-ex1F-6 | AGACTCCTCTGGCTGCTCT | 208 | ○ | 60 | / | Ⅰ | ○ |
| Exon 1 | CSS2-ex1R-6 | CAGCTCAGAGTCTCCAGGTT | ||||||
| Exon 1 | CSS2-ex1F-7 | GGCATCTCCCTGGGCTTCA | 227 | ○ | 60 | / | Ⅰ | ○ |
| Exon 1 | CSS2-ex1R-7 | GCAGGGTGGTAGGGCAAGA | ||||||
| Exon 1 | CSS2-ex1F-5 | GGGGAGAATTGGGAGCC | 218 | ○ | 60 | + | ||
| Exon 1 | CSS2-ex1R-5 | CAGGGCCATCGGAAGTTAT | ||||||
| Exon 2 | CSS2-ex2F-1 | CCTATCTGGGTGGGTATTGC | 499 | ○ | 60 | - | ||
| Exon 2 | CSS2-ex2R-1 | GAACCAGTCAAAGTCGTCGC | ||||||
| Exon 2 | CSS2-ex2F-2 | GACCCATTGGACACCTGC | 475 | ○ | 60 | + | ||
| Exon 2 | CSS2-ex2R-2 | ATTGGCATGGAAAAGCAGAC | ||||||
| Exon 2 | CSS2-ex3F | CCACAACAGGTCATAGGCTG | 397 | ○ | 60 | + | ||
| Exon 3 | CSS2-ex3R | CAGGGGCTGGGAAGTAGTTT | ||||||
| Exon 4 | CSS2-ex4F-1 | tactgcctacccagtcccc | 500 | ○ | 65 | + | ||
| Exon 4 | CSS2-ex4R-1 | AAGATCTCCACGCGGCTC | ||||||
| Exon 4 | CSS2-ex4F-2 | GGGTATGGAATACACGCTGG | 495 | ○ | 60 | + | ||
| Exon 4 | CSS2-ex4R-2 | AAGTCAGGCGTGAGCACC | ||||||
| Exon 4 | CSS2-ex4F-3 | ACTGCGCCTCATGGATCTAC | 459 | ○ | 60 | + | ||
| Exon 4 | CSS2-ex4R-3 | CGGTGGTACAGGTCCTCACT | ||||||
| Exon 4 | CSS2-ex4F-4 | TTCTCCAGTCTGCATGTGCT | 393 | ○ | 65 | - | ||
| Exon 4 | CSS2-ex4R-4 | ATGAGAAGTGGGTGGCTCTG |
連絡先
お問い合わせは、
山田（taka0197@kuhp.kyoto-u.ac.jp）宛