でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。
COL9A3
| Target | Primer name | Sense primer (5'->3') | Anti-sense primer (5'->3') | Product size [bp] | Annealing temperature[oC] |
|---|---|---|---|---|---|
| Exon1 | COL9A3-AF/AR | CGCCCTTGCTAACTCACAG | CAGCGAGGACCCATCGAG | 1284 | * |
| Exon2-3 | COL9A3-BF/BR | TTGGGTCTCACCGAGGA | CGGCAGGATGAGACAGGATA | 1211 | * |
| Exon4-5 | COL9A3-CF/CR | GCAAGCAGGACACACGAG | GACACAGAGCAGCCAGAGGT | 1011 | * |
| Exon6-8 | COL9A3-DF/DR | ACTTGGACCCTGTTGTCCTG | GAGGTCGGGTCTGGATCAT | 908 | * |
| Exon9 | COL9A3-QF/QR | CTTCCCCTCTCCCTTTTCC | TGGCCAAGTTAGGGTTAGGA | 400 | * |
| Exon10 | COL9A3-RF/RR | ACAGGATGAAGGGTCTCCAA | GTGCAGGTCTAGACGGTGGT | 400 | * |
| Exon11 | COL9A3-FF/FR | CGTCGACGTCCTGCTCTGT | CAGCCTAAATCACCCAGCTC | 814 | * |
| Exon12 | COL9A3-E12F2/E12R2 | GCAGGCCTCACTTCAGTGTTGC | AGCTGCTGTGCGGCAGCTTC | 154 | * |
| Exon13-14 | COL9A3-GF/GR | AGCTGCTTGGGCTTGAGTAG | GAGAACAGGGGCCCAGAG | 699 | * |
| Exon15 | COL9A3-HF/HR | TAGAGGAAGGGCTGCTTGTG | ACATAAGGACCCAGGGAACC | 670 | * |
| Exon16 | COL9A3-SF/SR | CTCCATGGTGTTAACTCTGTCC | AATTGGGTTCTTCCACATAAGG | 151 | * |
| Exon17-19 | COL9A3-IF/IR | CTGCCCACCATAGCTCCTT | ACTGAGCAGGCAGAAGCTG | 1214 | * |
| Exon20-22 | COL9A3-TF/TR | AATGTAACTGGCAGCCCTGA | CCAATGGCTCAGTGCTAACC | 456 | * |
| Exon23-24 | COL9A3-KF/KR | CTCCTGTGTCCACCCACTCT | GGTGGGGGACTGTGGTACAT | 403 | * |
| Exon25-26 | COL9A3-LF/LR | GGTGTTCACAGAAGCCCTTT | TGCAGATTTTGACCCTGTCC | 1138 | * |
| Exon27-29 | COL9A3-MF/MR | GCAGGTGTGTGGATGATTTG | GCACGTTGGCTTCTGTGTAG | 793 | * |
| Exon30 | COL9A3-NF/NR | ACCCCAAAGCAGTTAAACCA | ACCAGAAGCAGCGCCTACTA | 410 | * |
| Exon31 | COL9A3-OF/OR | GAAAAGCTGGCACCCTGTT | GCTTTGCCAAGAGAAGAGGA | 299 | * |
| Exon32 | COL9A3-PF/PR | AAGTCCCCCTGCTTTCAGTC | AGGGCCTTTTGAGGTATGCT | 448 | * |
*Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 95 oC for 30 sec annealing at 60 oC for 1 min extension at 72 oC for 1 min × 5 Cycles, and denaturatiion at 95 oC for 30 sec annealing at 55 oC for 1 min extention at 72 oC for 1 min × 35 Cycles.
| Target | Primer name | Sequence |
|---|---|---|
| Exon1 | COL9A3-AR (Reverse) | CAGCGAGGACCCATCGAG |
| Exon2 | COL9A3-2SR (Reverse) | GGCCTGGTTTTCTCTCCATCAG |
| Exon3 | COL9A3-3SF (Forward) | TCCTTCCAGTCTGTCCAGTTCAC |
| Exon4 | COL9A3-CSR (Reverse) | TGGAGCCCAAGATGACTAAG |
| Exon5 | COL9A3-CSF (Forward) | CTTGCTTGTCCAGGAATGAG |
| Exon6 | COL9A3-DF (Forward) | ACTTGGACCCTGTTGTCCTG |
| Exon7 | COL9A3-DSF (Forward) | CAGCCCTTCTTGTGCCTG |
| Exon8 | COL9A3-DR (Reverse) | GAGGTCGGGTCTGGATCAT |
| Exon9 | COL9A3-QF (Forward) | CTTCCCCTCTCCCTTTTCC |
| Exon10 | COL9A3-RF (Forward) | ACAGGATGAAGGGTCTCCAA |
| Exon11 | COL9A3-FSR2 (Reverse) | AGCACAGGGTGGTGCTTG |
| Exon12 | COL9A3-E12F2 (Forward) | GCAGGCCTCACTTCAGTGTTGC |
| Exon13 | COL9A3-GF (Forward) | AGCTGCTTGGGCTTGAGTAG |
| Exon14 | COL9A3-GR (Reverse) | GAGAACAGGGGCCCAGAG |
| Exon15 | COL9A3-HF (Forward) | TAGAGGAAGGGCTGCTTGTG |
| Exon16 | COL9A3-SF (Forward) | CTCCATGGTGTTAACTCTGTCC |
| Exon16 | COL9A3-SR (Reverse) | AATTGGGTTCTTCCACATAAGG |
| Exon17 | COL9A3-IF (Forward) | CTGCCCACCATAGCTCCTT |
| Exon18 | COL9A3-IR (Reverse) | ACTGAGCAGGCAGAAGCTG |
| Exon19 | COL9A3-IR (Reverse) | ACTGAGCAGGCAGAAGCTG |
| Exon20 | COL9A3-TSR (Reverse) | CTGCCCTGTAGAGGAAGGAG |
| Exon21 | COL9A3-TR (Reverse) | CCAATGGCTCAGTGCTAACC |
| Exon22 | COL9A3-TR (Reverse) | CCAATGGCTCAGTGCTAACC |
| Exon23 | COL9A3-KF (Forward) | CTCCTGTGTCCACCCACTCT |
| Exon24 | COL9A3-KR (Reverse) | GGTGGGGGACTGTGGTACAT |
| Exon25 | COL9A3-LF (Forward) | GGTGTTCACAGAAGCCCTTT |
| Exon26 | COL9A3-LR (Reverse) | TGCAGATTTTGACCCTGTCC |
| Exon27 | COL9A3-MSR (Reverse) | CGTGATGCCAGGAACACC |
| Exon28 | COL9A3-MSR (Reverse) | CGTGATGCCAGGAACACC |
| Exon28 | COL9A3-MSF (Forward) | CACCTTCCTCTGTTCCTCTG |
| Exon29 | COL9A3-MSF (Forward) | CACCTTCCTCTGTTCCTCTG |
| Exon30 | COL9A3-NF (Forward) | ACCCCAAAGCAGTTAAACCA |
| Exon31 | COL9A3-OR (Reverse) | GCTTTGCCAAGAGAAGAGGA |
| Exon32 | COL9A3-PF (Forward) | AAGTCCCCCTGCTTTCAGTC |
連絡先
お問い合わせは、
山田（taka0197@med.hokudai.ac.jp）宛