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COL9A2

COL9A2 PCR Primer
TargetPrimer nameSense primer (5'->3')Anti-sense primer (5'->3')Product size [bp]Annealing temperature[oC]
Exon1COL9A2-E1F/E1RTCCTCTCAGGTGACAGCTCACCGATGGGCACCATGTATGCAC 410*
Exon2COL9A2-E2F/E2RTGTAATGGCACACAGTGCCTGCACCACAGCGCTCACAAAGTTCTC309*
Exon3-4COL9A2-i2i4/F / i2i4/RTAGGGGACCTGGACAGAAGACAATGACCCTGGGTGAGAGT496*
Exon5-7COL9A2-E5-7F/E5-7RTTGAACCTGCCCTGCTCTGTGACTATCACAGCAAGCTGGCTCC514*
Exon8COL9A2-E8F/E8RGTGCCTGTCTCTACCTTTGTGCGGAGATGAAGGCCTGATTGACAG341*
Exon9-10COL9A2-E9-10F/E9-10RCAAGATGGTGGATCTCACAAAGTGAC CCACAGGGTCCTGTGATCAG544*
Exon11-12COL9A2-E11-12F/E11-12RACTGCCCAGCTGATCACAGGTGTAGGACCATCTCCACGTATCC293*
Exon13COL9A2-E13F/E13RTGTCTCTGGTCATGTCTCCTTGCTACAAGCTAGAGGCCTGAGCAG319*
Exon14-16COL9A2-E14-16F/E14-16RGGCATCAGACAAGGTATCATGAAGTGTGCCTTGTCCTGCCCAGTACTC511*
Exon17COL9A2-E17F/E17RAGGCCACTGTTCTCAGTGGTTCCAGTTGCAGAGTTCAGACTAGCATC249*
Exon18COL9A2-E18F/E18RACACTGCAGCTGTCTCAGAGGTGTCTAGAATCCAGGACTCAAGGTC210*
Exon19COL9A2-E19F/E19RTCTCAGTTTCCCTACCTGCATCTGACAAGGCAAGAGGTGGTGATTGAG248*
Exon20COL9A2-E20F/E20RCTGGACCTTCTGTTGTTCAGCTCAGGCTGAGGAGTGCATGATGG 338*
Exon21COL9A2-E21F/E21RTCAGGAGCCAAGAGGATTGAACCAGGGCAGAGCCAGACTAACTC304*
Exon22COL9A2-E22F/E22RAACCATGGAACAGGTGGTCTGGGAGCAGATGCTGTATCCAGCAG267*
Exon23-24COL9A2-E23-24F/E23-24RCTGCCAGTGACAGCAGGAATGAAGGATCTGAGTCCTGCCGAC402*
Exon25-27COL9A2-E25-27F/E25-27RGGTGTGGCCGAAAGTTAGGTGGCCCAGGAAAGTCGGAGAAC409*
Exon28COL9A2-E28F/E28RCGTTCTCCGACTTTCCTGGGACAGCCTTCCTGTCTGCTCTG403*
Exon29COL9A2-E29F/E29RCCAGGTACAATCTGTTCCTCAGCAGACAAGTGAACACGTGGAGGC288*
Exon30COL9A2-E30F/E30RCCCTAGTGTTACTGCTGACACCGAAGGGTCTGTATGTCATCCTGAG355*
Exon31COL9A2-E31F/E31RAAGCCCTGCGTGACCATTTCCAGAAGCTGGGTATCAAGGACTGAG347*
Exon32COL9A2-E32F/E32RTCTGGGCCTGAGACAAGAGGTGATGGTTTCCTGGACTGGGGATG349*

*Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 95 oC for 30 sec annealing at 60 oC for 1 min extension at 72 oC for 1 min × 5 Cycles.

COL9A2 Sequence Primer
TargetPrimer nameSequence
Exon1COL9A2-E1R (Reverse)CGATGGGCACCATGTATGCAC
Exon2COL9A2-E2R (Reverse)ACCACAGCGCTCACAAAGTTCTC
Exon3COL9A2-i2i4SF (Forward)GGACCTGGACAGAAGAGAGG
Exon4COL9A2-i2i4SF (Forward)GGACCTGGACAGAAGAGAGG
Exon5COL9A2-E5-7R (Reverse)ACTATCACAGCAAGCTGGCTCC
Exon6COL9A2-E5-7R (Reverse)ACTATCACAGCAAGCTGGCTCC
Exon7COL9A2-E5-7R (Reverse)ACTATCACAGCAAGCTGGCTCC
Exon8COL9A2-E8F (Forward)GTGCCTGTCTCTACCTTTGTGC
Exon9COL9A2-E9-10F (Forward)CAAGATGGTGGATCTCACAAAGTGAC
Exon10COL9A2-E9-10F (Forward)CAAGATGGTGGATCTCACAAAGTGAC
Exon11COL9A2-E11-12R (Reverse)TGTAGGACCATCTCCACGTATCC
Exon12COL9A2-E11-12R (Reverse)TGTAGGACCATCTCCACGTATCC
Exon13COL9A2-E13F (Forward)TGTCTCTGGTCATGTCTCCTTGC
Exon14COL9A2-E14-16F (Forward)GGCATCAGACAAGGTATCATGAAGTG
Exon15COL9A2-E14-16F (Forward)GGCATCAGACAAGGTATCATGAAGTG
Exon16COL9A2-E14-16F (Forward)GGCATCAGACAAGGTATCATGAAGTG
Exon17COL9A2-E17R (Reverse)CAGTTGCAGAGTTCAGACTAGCATC
Exon18COL9A2-E18F (Forward)ACACTGCAGCTGTCTCAGAGG
Exon19COL9A2-E19F (Forward)TCTCAGTTTCCCTACCTGCATCTG
Exon20COL9A2-E20F (Forward)CTGGACCTTCTGTTGTTCAGCTC
Exon21COL9A2-E21R (Reverse)AGGGCAGAGCCAGACTAACTC
Exon22COL9A2-E22F (Forward)AACCATGGAACAGGTGGTCTGG
Exon23COL9A2-E23-24F (Forward)CTGCCAGTGACAGCAGGAATG
Exon24COL9A2-E23-24F (Forward)CTGCCAGTGACAGCAGGAATG
Exon25COL9A2-E25-27F (Forward)GGTGTGGCCGAAAGTTAGGTG
Exon26COL9A2-E25-27F (Forward)GGTGTGGCCGAAAGTTAGGTG
Exon27COL9A2-E25-27F (Forward)GGTGTGGCCGAAAGTTAGGTG
Exon28COL9A2-E28R (Reverse)ACAGCCTTCCTGTCTGCTCTG
Exon29COL9A2-E29F (Forward)CCAGGTACAATCTGTTCCTCAGC
Exon30COL9A2-E30R (Reverse)GAAGGGTCTGTATGTCATCCTGAG
Exon31COL9A2-E31R (Reverse)AGAAGCTGGGTATCAAGGACTGAG
Exon32COL9A2-E32R (Reverse)ATGGTTTCCTGGACTGGGGATG
管理・運営：骨系統疾患コンソーシウム
連絡先

お問い合わせは、
山田（taka0197@med.hokudai.ac.jp）宛

でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。