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COL9A1

COL9A1 PCR Primer
TargetPrimer nameSense primer (5'->3')Anti-sense primer (5'->3')Product size [bp]Annealing temperature[oC]
Exon1COL9A1-E1F/E1RAGAACACATAGTCCTAGGGTAACAGTGTCACCTACTCTCATCTTACCACTGC226*1
Exon2COL9A1-E2F2/E2R2GGTTTACTAGGTTTGAAAATATGGAAACTCAGTCTTCAGTCAGCCACA240*1
Exon3-4COL9A1-E3-4F/E3-4RCAAACACTTTACCTCTAAGCAAGCTGGCAGTATTAAGGTACTGAAGATAGGCAGG717*1
Exon5COL9A1-E5F/E5RAGGTCTGGCATCTAAAATGGCCTCATCTGGGACATGCTGCACTCAG600*1
Exon6COL9A1-E6F/E6RTCCAGTGTGCTCAACCAGTTCAAGAGGCTCTCTTAGCGAAAGAGAGTG337*1
Exon7COL9A1-E7F/E7RGGCTCAAGTAAGTTGCGATCGAGGGCTCTGGACTTCGATAGGAC259*1
Exon8-10COL9A1-i7i10PCRF/i7i10PCRRGAGACATTTTTGCGTTGGGTGCCAAGTTTAGAGCCACAGC929*1
Exon11COL9A1-E11F/E11RATGCAGTTAGCAGGAAGTTAGGAGATGTTAGTTGGCTTGCAAACACTGC380*1
Exon12COL9A1-E12F2/E12R2CCATAATGGGGTGTTTTAATGCAAATGCTTACCATACCAGATGTCA331*1
Exon13COL9A1-E13F/E13RTTCCTGTAGAAGCAGAGCCGTGCACTGAGTAGACCGTTAGTAGGAG291*2
Exon14COL9A1-E14F/E14RACCTGCAAGATCTTAGGTGGCTACTGAGGACGACACTCTCTTACTTGAC554*1
Exon15COL9A1-E15F/E15RTGGCAGGTCTATGCATGGCTGCAACCCTGACTCTCAGACACAAAC233*1
Exon16COL9A1-E16F/E16RAGAGAACATGCCCATGAGTCAGGACTAGCGTTACAGACTGCTCTGC351*2
Exon17COL9A1-E17F/E17RAGATACTGTCACAAAGCGGGAGTCTCTCTGCACCAGGAAGGCTAATGC226*1
Exon18COL9A1-E18F/E18RGAGCCTTCTGATCACTTTCTGTACCTGGTGAAGTGGAACAACCGTCTG452*1
Exon19COL9A1-E19F/E19RGTTCGTTTGCTAGAATAACATTTGGTGTGCGACACCAAGTTCATGTAATGCTATTCATCC227*1
Exon20COL9A1-E20F2/E20R2TCTAGATCAGCTTTTGTGATGAGATATGTTTAAACGGCCAAAAT206*1
Exon21COL9A1-E21F/E21RACAAGTTTACCTGAGGTGCCTGAGCAATACTGCACACACATGCATACATAGC300*1
Exon22-24COL9A1-E22-24F/E22-24RGGAAGACTAATAGTTTGGTGACGTGGGTAATGGAAATGCCACACTCAGTTGG606*1
Exon25COL9A1-E25F/E25RACGTCTGTGCTCCTCCGTTTCATCACACTGGCCTACAGCTTAGG435*1
Exon26COL9A1-E26F/E26RGTTATCATGATTCCCTCAGCCTCGTCAAAGTATGCTTTCTGTAGCTAGG337*1
Exon27-28COL9A1-E27-28F/E27-28RCAGTCTGGGTAAGGCAGATTGATGGGATGAATGAATGGAAGAACATCCAGC345*1
Exon29COL9A1-E29F/E29RAGGATGGCCTATGCCTTTAGCTGGCAGCACTTCACATTTCACCAGG414*1
Exon30COL9A1-E30F/E30RAGGTTGTTTCTGCCTTCCGTGCCTCAACATGGTAGAAATCACCCTTCC206*1
Exon31COL9A1-E31F/E31RTCCCATCACATTATCCGTACTTGTGCTGCGGACCAAACTCAACACCAG310*2
Exon32COL9A1-E32F/E32RGACTAACCACTGACAGCTAGATTGGGCAGTGAGGATAACAGTGTCCAG353*2
Exon33COL9A1-E33F/E33RGTGCCGGAGTTAATCTGTTCATGGCAAGATTGCACCACTGCATTCACG323*1
Exon34-35COL9A1-E34-35F/E34-35RACCTGTTAGGATGCAGAGTGCAGCCCTAAATGTCCTATCTTTAAGGCACAC647*1
Exon36COL9A1-E36F/E36RTCTGGCAGAAAGCCCACAGAGACTGTTGTGGTAGAGCAGGGTC558*1
Exon37COL9A1-E37F/E37RGTGTTTAGGAAGTGGCCATTGGTTCCAATTCAGGCTGTGTACTTGCTACC438*2
Exon38COL9A1-E38F/E38RCCTCTGCCAGTTTGCATCAATGAGCCAGCTTTGGATGGTGTTTCTCAC328*1

*1 Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 95 oC for 30 sec annealing at 60 oC for 1 min extension at 72 oC for 1 min × 5 Cycles, and denaturatiion at 95 oC for 30 sec annealing at 55 oC for 1 min extention at 72 oC for 1 min × 35 Cycles.

*2 Applied Biosystems TaqGold PCR system used initial denaturation at 95oC 10 min denaturation 94 oC 1min annealing at 58 oC 1min extension at 72 oC ×35 cycles.

COL9A1 Sequence Primer
TargetPrimer nameSequence
Exon1COL9A1-E1R (Reverse)TCACCTACTCTCATCTTACCACTGC
Exon2COL9A1-E2F2 (Forward)GGTTTACTAGGTTTGAAAATATGGAA
Exon3COL9A1-E3-4F (Forward)CAAACACTTTACCTCTAAGCAAGCTGG
Exon4COL9A1-E3-4R (Reverse)CAGTATTAAGGTACTGAAGATAGGCAGG
Exon5COL9A1-E5F (Forward)AGGTCTGGCATCTAAAATGGCCTC
Exon5COL9A1-E5R (Reverse)ATCTGGGACATGCTGCACTCAG
Exon6COL9A1-E6F (Forward)TCCAGTGTGCTCAACCAGTTCAAG
Exon7COL9A1-E7F (Forward)GGCTCAAGTAAGTTGCGATCGAG
Exon8COL9A1-i7i10SF2 (Forward)GGCCTCCTCTGGAAGGTAAC
Exon9COL9A1-i7i10SEQF1 (Forward)CCCACTTAAAGGCAGGATCA
Exon10COL9A1-i7i10SEQF1 (Forward)CCCACTTAAAGGCAGGATCA
Exon11COL9A1-E11F (Forward)ATGCAGTTAGCAGGAAGTTAGGAG
Exon12COL9A1-E12F2 (Forward)CCATAATGGGGTGTTTTAATGC
Exon13COL9A1-E13F (Forward)TTCCTGTAGAAGCAGAGCCGTG
Exon14COL9A1-E14R (Reverse)TGAGGACGACACTCTCTTACTTGAC
Exon15COL9A1-E15R (Reverse)CAACCCTGACTCTCAGACACAAAC
Exon16COL9A1-E16F (Forward)AGAGAACATGCCCATGAGTCAGG
Exon17COL9A1-E17F (Forward)AGATACTGTCACAAAGCGGGAGTC
Exon18COL9A1-E18F (Forward)GAGCCTTCTGATCACTTTCTGTACC
Exon19COL9A1-E19R (Reverse)GACACCAAGTTCATGTAATGCTATTCATCC
Exon20COL9A1-E20F2 (Forward)TCTAGATCAGCTTTTGTGATGAGA
Exon21COL9A1-E21F (Forward)ACAAGTTTACCTGAGGTGCCTGAG
Exon22COL9A1-E22-24R (Reverse)GTAATGGAAATGCCACACTCAGTTGG
Exon23COL9A1-E22-24R (Reverse)GTAATGGAAATGCCACACTCAGTTGG
Exon24COL9A1-E22-24R (Reverse)GTAATGGAAATGCCACACTCAGTTGG
Exon25COL9A1-E25R (Reverse)ATCACACTGGCCTACAGCTTAGG
Exon26COL9A1-E26R (Reverse)GTCAAAGTATGCTTTCTGTAGCTAGG
Exon27COL9A1-E27-28F (Forward)CAGTCTGGGTAAGGCAGATTGATG
Exon28COL9A1-E27-28F (Forward)CAGTCTGGGTAAGGCAGATTGATG
Exon29COL9A1-E29F (Forward)AGGATGGCCTATGCCTTTAGCTG
Exon30COL9A1-E30F (Forward)AGGTTGTTTCTGCCTTCCGTGC
Exon31COL9A1-E31F (Forward)TCCCATCACATTATCCGTACTTGTGC
Exon32COL9A1-E32F (Forward)GACTAACCACTGACAGCTAGATTGG
Exon33COL9A1-E33F (Forward)GTGCCGGAGTTAATCTGTTCATGG
Exon34COL9A1-E34-35R (Reverse)CCCTAAATGTCCTATCTTTAAGGCACAC
Exon35COL9A1-E34-35R (Reverse)CCCTAAATGTCCTATCTTTAAGGCACAC
Exon36COL9A1-E36R (Reverse)ACTGTTGTGGTAGAGCAGGGTC
Exon37COL9A1-E37R (Reverse)CAATTCAGGCTGTGTACTTGCTACC
Exon38COL9A1-E38R (Reverse)CCAGCTTTGGATGGTGTTTCTCAC
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連絡先

お問い合わせは、
遺伝子解析、DNA診断に関するもの:
山田(taka0197@med.hokudai.ac.jp)宛

診断/X線診断に関するもの:
西村 (gen-n@pc4.so-net.ne.jp)宛
治療に関するもの:
池川(sikegawa@ims.u-tokyo.ac.jp)宛

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