でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。
COL9A1
| Target | Primer name | Sense primer (5'->3') | Anti-sense primer (5'->3') | Product size [bp] | Annealing temperature[oC] |
|---|---|---|---|---|---|
| Exon1 | COL9A1-E1F/E1R | AGAACACATAGTCCTAGGGTAACAGTG | TCACCTACTCTCATCTTACCACTGC | 226 | *1 |
| Exon2 | COL9A1-E2F2/E2R2 | GGTTTACTAGGTTTGAAAATATGGAA | ACTCAGTCTTCAGTCAGCCACA | 240 | *1 |
| Exon3-4 | COL9A1-E3-4F/E3-4R | CAAACACTTTACCTCTAAGCAAGCTGG | CAGTATTAAGGTACTGAAGATAGGCAGG | 717 | *1 |
| Exon5 | COL9A1-E5F/E5R | AGGTCTGGCATCTAAAATGGCCTC | ATCTGGGACATGCTGCACTCAG | 600 | *1 |
| Exon6 | COL9A1-E6F/E6R | TCCAGTGTGCTCAACCAGTTCAAG | AGGCTCTCTTAGCGAAAGAGAGTG | 337 | *1 |
| Exon7 | COL9A1-E7F/E7R | GGCTCAAGTAAGTTGCGATCGAG | GGCTCTGGACTTCGATAGGAC | 259 | *1 |
| Exon8-10 | COL9A1-i7i10PCRF/i7i10PCRR | GAGACATTTTTGCGTTGGGT | GCCAAGTTTAGAGCCACAGC | 929 | *1 |
| Exon11 | COL9A1-E11F/E11R | ATGCAGTTAGCAGGAAGTTAGGAG | ATGTTAGTTGGCTTGCAAACACTGC | 380 | *1 |
| Exon12 | COL9A1-E12F2/E12R2 | CCATAATGGGGTGTTTTAATGC | AAATGCTTACCATACCAGATGTCA | 331 | *1 |
| Exon13 | COL9A1-E13F/E13R | TTCCTGTAGAAGCAGAGCCGTG | CACTGAGTAGACCGTTAGTAGGAG | 291 | *2 |
| Exon14 | COL9A1-E14F/E14R | ACCTGCAAGATCTTAGGTGGCTAC | TGAGGACGACACTCTCTTACTTGAC | 554 | *1 |
| Exon15 | COL9A1-E15F/E15R | TGGCAGGTCTATGCATGGCTG | CAACCCTGACTCTCAGACACAAAC | 233 | *1 |
| Exon16 | COL9A1-E16F/E16R | AGAGAACATGCCCATGAGTCAGG | ACTAGCGTTACAGACTGCTCTGC | 351 | *2 |
| Exon17 | COL9A1-E17F/E17R | AGATACTGTCACAAAGCGGGAGTC | TCTCTGCACCAGGAAGGCTAATGC | 226 | *1 |
| Exon18 | COL9A1-E18F/E18R | GAGCCTTCTGATCACTTTCTGTACC | TGGTGAAGTGGAACAACCGTCTG | 452 | *1 |
| Exon19 | COL9A1-E19F/E19R | GTTCGTTTGCTAGAATAACATTTGGTGTGC | GACACCAAGTTCATGTAATGCTATTCATCC | 227 | *1 |
| Exon20 | COL9A1-E20F2/E20R2 | TCTAGATCAGCTTTTGTGATGAGA | TATGTTTAAACGGCCAAAAT | 206 | *1 |
| Exon21 | COL9A1-E21F/E21R | ACAAGTTTACCTGAGGTGCCTGAG | CAATACTGCACACACATGCATACATAGC | 300 | *1 |
| Exon22-24 | COL9A1-E22-24F/E22-24R | GGAAGACTAATAGTTTGGTGACGTGG | GTAATGGAAATGCCACACTCAGTTGG | 606 | *1 |
| Exon25 | COL9A1-E25F/E25R | ACGTCTGTGCTCCTCCGTTTC | ATCACACTGGCCTACAGCTTAGG | 435 | *1 |
| Exon26 | COL9A1-E26F/E26R | GTTATCATGATTCCCTCAGCCTC | GTCAAAGTATGCTTTCTGTAGCTAGG | 337 | *1 |
| Exon27-28 | COL9A1-E27-28F/E27-28R | CAGTCTGGGTAAGGCAGATTGATG | GGATGAATGAATGGAAGAACATCCAGC | 345 | *1 |
| Exon29 | COL9A1-E29F/E29R | AGGATGGCCTATGCCTTTAGCTG | GCAGCACTTCACATTTCACCAGG | 414 | *1 |
| Exon30 | COL9A1-E30F/E30R | AGGTTGTTTCTGCCTTCCGTGC | CTCAACATGGTAGAAATCACCCTTCC | 206 | *1 |
| Exon31 | COL9A1-E31F/E31R | TCCCATCACATTATCCGTACTTGTGC | TGCGGACCAAACTCAACACCAG | 310 | *2 |
| Exon32 | COL9A1-E32F/E32R | GACTAACCACTGACAGCTAGATTGG | GCAGTGAGGATAACAGTGTCCAG | 353 | *2 |
| Exon33 | COL9A1-E33F/E33R | GTGCCGGAGTTAATCTGTTCATGG | CAAGATTGCACCACTGCATTCACG | 323 | *1 |
| Exon34-35 | COL9A1-E34-35F/E34-35R | ACCTGTTAGGATGCAGAGTGCAG | CCCTAAATGTCCTATCTTTAAGGCACAC | 647 | *1 |
| Exon36 | COL9A1-E36F/E36R | TCTGGCAGAAAGCCCACAGAG | ACTGTTGTGGTAGAGCAGGGTC | 558 | *1 |
| Exon37 | COL9A1-E37F/E37R | GTGTTTAGGAAGTGGCCATTGGTTC | CAATTCAGGCTGTGTACTTGCTACC | 438 | *2 |
| Exon38 | COL9A1-E38F/E38R | CCTCTGCCAGTTTGCATCAATGAG | CCAGCTTTGGATGGTGTTTCTCAC | 328 | *1 |
*1 Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 95 oC for 30 sec annealing at 60 oC for 1 min extension at 72 oC for 1 min × 5 Cycles, and denaturatiion at 95 oC for 30 sec annealing at 55 oC for 1 min extention at 72 oC for 1 min × 35 Cycles.
*2 Applied Biosystems TaqGold PCR system used initial denaturation at 95oC 10 min denaturation 94 oC 1min annealing at 58 oC 1min extension at 72 oC ×35 cycles.
| Target | Primer name | Sequence |
|---|---|---|
| Exon1 | COL9A1-E1R (Reverse) | TCACCTACTCTCATCTTACCACTGC |
| Exon2 | COL9A1-E2F2 (Forward) | GGTTTACTAGGTTTGAAAATATGGAA |
| Exon3 | COL9A1-E3-4F (Forward) | CAAACACTTTACCTCTAAGCAAGCTGG |
| Exon4 | COL9A1-E3-4R (Reverse) | CAGTATTAAGGTACTGAAGATAGGCAGG |
| Exon5 | COL9A1-E5F (Forward) | AGGTCTGGCATCTAAAATGGCCTC |
| Exon5 | COL9A1-E5R (Reverse) | ATCTGGGACATGCTGCACTCAG |
| Exon6 | COL9A1-E6F (Forward) | TCCAGTGTGCTCAACCAGTTCAAG |
| Exon7 | COL9A1-E7F (Forward) | GGCTCAAGTAAGTTGCGATCGAG |
| Exon8 | COL9A1-i7i10SF2 (Forward) | GGCCTCCTCTGGAAGGTAAC |
| Exon9 | COL9A1-i7i10SEQF1 (Forward) | CCCACTTAAAGGCAGGATCA |
| Exon10 | COL9A1-i7i10SEQF1 (Forward) | CCCACTTAAAGGCAGGATCA |
| Exon11 | COL9A1-E11F (Forward) | ATGCAGTTAGCAGGAAGTTAGGAG |
| Exon12 | COL9A1-E12F2 (Forward) | CCATAATGGGGTGTTTTAATGC |
| Exon13 | COL9A1-E13F (Forward) | TTCCTGTAGAAGCAGAGCCGTG |
| Exon14 | COL9A1-E14R (Reverse) | TGAGGACGACACTCTCTTACTTGAC |
| Exon15 | COL9A1-E15R (Reverse) | CAACCCTGACTCTCAGACACAAAC |
| Exon16 | COL9A1-E16F (Forward) | AGAGAACATGCCCATGAGTCAGG |
| Exon17 | COL9A1-E17F (Forward) | AGATACTGTCACAAAGCGGGAGTC |
| Exon18 | COL9A1-E18F (Forward) | GAGCCTTCTGATCACTTTCTGTACC |
| Exon19 | COL9A1-E19R (Reverse) | GACACCAAGTTCATGTAATGCTATTCATCC |
| Exon20 | COL9A1-E20F2 (Forward) | TCTAGATCAGCTTTTGTGATGAGA |
| Exon21 | COL9A1-E21F (Forward) | ACAAGTTTACCTGAGGTGCCTGAG |
| Exon22 | COL9A1-E22-24R (Reverse) | GTAATGGAAATGCCACACTCAGTTGG |
| Exon23 | COL9A1-E22-24R (Reverse) | GTAATGGAAATGCCACACTCAGTTGG |
| Exon24 | COL9A1-E22-24R (Reverse) | GTAATGGAAATGCCACACTCAGTTGG |
| Exon25 | COL9A1-E25R (Reverse) | ATCACACTGGCCTACAGCTTAGG |
| Exon26 | COL9A1-E26R (Reverse) | GTCAAAGTATGCTTTCTGTAGCTAGG |
| Exon27 | COL9A1-E27-28F (Forward) | CAGTCTGGGTAAGGCAGATTGATG |
| Exon28 | COL9A1-E27-28F (Forward) | CAGTCTGGGTAAGGCAGATTGATG |
| Exon29 | COL9A1-E29F (Forward) | AGGATGGCCTATGCCTTTAGCTG |
| Exon30 | COL9A1-E30F (Forward) | AGGTTGTTTCTGCCTTCCGTGC |
| Exon31 | COL9A1-E31F (Forward) | TCCCATCACATTATCCGTACTTGTGC |
| Exon32 | COL9A1-E32F (Forward) | GACTAACCACTGACAGCTAGATTGG |
| Exon33 | COL9A1-E33F (Forward) | GTGCCGGAGTTAATCTGTTCATGG |
| Exon34 | COL9A1-E34-35R (Reverse) | CCCTAAATGTCCTATCTTTAAGGCACAC |
| Exon35 | COL9A1-E34-35R (Reverse) | CCCTAAATGTCCTATCTTTAAGGCACAC |
| Exon36 | COL9A1-E36R (Reverse) | ACTGTTGTGGTAGAGCAGGGTC |
| Exon37 | COL9A1-E37R (Reverse) | CAATTCAGGCTGTGTACTTGCTACC |
| Exon38 | COL9A1-E38R (Reverse) | CCAGCTTTGGATGGTGTTTCTCAC |
連絡先
お問い合わせは、
山田（taka0197@med.hokudai.ac.jp）宛