でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。
COL2A1
| Target | Primer name | Sense primer (5'->3') | Anti-sense primer (5'->3') | Product size [bp] | Annealing temperature[oC] |
|---|---|---|---|---|---|
| Exon1 | COL2A1_E1F/E1R | AGAAGACGCAGAGCGCTGCT | ACAGAGTCTTGATTGGCAG | 437 | * |
| Exon2 | COL2A1_E2F/E2R | GCCTACATTCTTCAGCATT | TCTAGTGTCTCAGGCTCATTCACT | 521 | * |
| Exon3 | COL2A1_E3F2/E3R | TCAGTGGAGTCACAGGAAGCTG | CTGGATTAACATAGCATTGC | 242 | * |
| Exon4-5 | COL2A1_E4-5F/E4-5R | GCAATGCTATGTTAATCCAG | TCACCTTTGTCACCACGATC | 478 | * |
| Exon6-7 | COL2A1_E6-7F/E6-7R | GCTCTCTCTCACAGATTGTA | GTAAGTGCAAGCAGCAATT | 548 | * |
| Exon8 | COL2A1_E8F2/E8R2 | CCAGGCATCAGTGGTAATTC | TTCTTAGTCACTCCAGCGCA | 442 | * |
| Exon9-10 | COL2A1_E9-10F/E9-10R | GCAGACAGCAGCTACTGTTCT | ACAGCAGCTGCGTCCTAGT | 624 | * |
| Exon11 | COL2A1_E11F/E11R | AATCCCACTATGATCTCTGC | CAGTCTTGCTCCTCAAGATAC | 329 | * |
| Exon12 | COL2A1_E12F/E12R | CAGGATGTCTACAAAGGATGC | CAAAGTGGAGGTGTTCAGAG | 816 | * |
| Exon13 | COL2A1_E13-14F/E13-14R | GTCACTTCTGAGATGAAACGCC | TCAGCTCGCACTGACACAAAC | 745 | * |
| Exon15 | COL2A1_E15F2/E15R2 | TCCTCAGAACAGCCTTGGCT | TTACAAGCACACCTTGCTCC | 537 | * |
| Exon16 | COL2A1_E16SF/E16R2 | ACCATCAGGAGGTCCTTGTT | GGGATTACCTGATAATCTGG | 445 | * |
| Exon17 | COL2A1_E17F/E17R | TCGCACAGACACCAAAACTGCA | ATACACCCTCGAGACTGCCTTG | 291 | * |
| Exon18 | COL2A1_E18F/E18R | CTTCTCACCACAGATGTAGTCA | GTTGTTGAGGGAGCAATGAGCAAG | 368 | * |
| Exon19 | COL2A1_E19F/E19R | AACTGTGTGTGAACCGACATGTTC | CAGAATGAAGGTTTGGTGGTTG | 365 | * |
| Exon20-22 | COL2A1_E20-22F/E19-21R | CTTGAAACACATAGTGGGAATGTC | GACTCCAGAGATGTCAGTGGAAC | 415 | * |
| Exon20-22 | COL2A1_E20-22F/E20-22R | CTTGAAACACATAGTGGGAATGTC | CTGTGCCTCATAGAACAGCAG | 666 | * |
| Exon23 | COL2A1_E23F/E23R | GTGAACAGTTGGATCTTTAG | CAGAACACGGACCACAAGGACT | 380 | * |
| Exon24-26 | COL2A1_E24-26F/E24-26R | CAGAAGTTAACCTCTGAGAATC | CATCTCTCTTTTCCCTTGCTTC | 649 | * |
| Exon27-28 | COL2A1_E27-28F/E27-28R | TAGATGCTGAGCATGTGTGG | GATCAACACTCAATACTGAGG | 787 | * |
| Exon29-30 | COL2A1_E29-30F/E29-30R | AGTACCTTTGCCCAATCCTA | CGAAGTGACAAGCGTTAGCA | 774 | * |
| Exon31-33 | COL2A1_E31-33F/E31-33R | CTGGACAGCAGCAGGCACTATC | GACTCCAGGCTACCACGAAG | 828 | * |
| Exon34 | COL2A1_E34-35F/E34-35R | CGGGCTCAGGAGGAATGAAG | GATCCTAATGCCCAGCAGTC | 711 | * |
| Exon35 | COL2A1_E35F2/E35R2 | GTGACATCCTTTCCCCTGTT | AAGGTGTGGAGAGGAAAGGA | 599 | * |
| Exon36-37 | COL2A1_E36-37F/E36-37R | GTCTGGGCAGTCTGCCACTG | GCAGGCAGAGGCTCTGTTAA | 928 | * |
| Exon38-39 | COL2A1_E38-39F/E38-39R | GCACGTCACTCCCATCATGT | CAGGAGATCAGCAGCTTGGT | 840 | * |
| Exon40-41 | COL2A1_E40-41F/E40-41R | ACCAAGCTGCTGATCTCCTG | TCAGTTAGCTACTCCTCCAG | 976 | * |
| Exon42-43 | COL2A1_E42-43F/E42-43R | TGTCTCACATGGTGAGAAGGTTG | CTTCAGGAGAGGGCAGACAAG | 761 | * |
| Exon44-45 | COL2A1_E44-45F/E44-45R | CAAGGCTTCTACCTCCCTCA | TCTAGGCTGAGATGAGACTTGTTC | 751 | * |
| Exon46-47 | COL2A1_E46-47F/E46-47R | GTTGGAACAAGTCTCATCTCA | GACAGACACCGATTGAGTCAGGTCA | 591 | * |
| Exon48-49 | COL2A1_E48-49F/E48-49R | TCCGCAGTCTCTGGACTAAG | TCTGTCTGACAGCGAGAGGCT | 960 | * |
| Exon50-51 | COL2A1_E50-51F/E50-51R | ACTGAGCATGTGAAGAACTG | AACCCTCTGGCGGAAACTTC | 1007 | * |
| Exon52-53 | COL2A1_E52-53F/E52-53R | CTTAGGGCTGGACTTAGCTC | TCTCTCACCTGTCACTCAGC | 1131 | * |
| Exon54 | COL2A1_E54F/E54R | TGGAGAGGCCTTTGGCAAGC | TCAGGTCAGCCATTCAGTGC | 417 | * |
*Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 94 oC for 30 sec annealing at 60 oC for 1 min extension at 72 oC for 1 min × 5 Cycles, and denaturatiion at 95 oC for 30 sec annealing at 55 oC for 1 min extention at 72 oC for 1 min × 35 Cycles.
| Target | Primer name | Sequence |
|---|---|---|
| Exon1 | COL2A1_E1F | AGAAGACGCAGAGCGCTGCT |
| Exon2 | COL2A1_E2F | GCCTACATTCTTCAGCATT |
| Exon3 | COL2A1_E3R | CTGGATTAACATAGCATTGC |
| Exon4-5 | COL2A1_E4-5F | GCAATGCTATGTTAATCCAG |
| Exon6-7 | COL2A1_E6-7R | GTAAGTGCAAGCAGCAATT |
| Exon8 | COL2A1_E8F2 | CCAGGCATCAGTGGTAATTC |
| Exon9-10 | COL2A1_E9-10R | ACAGCAGCTGCGTCCTAGT |
| Exon11 | COL2A1_E11F | AATCCCACTATGATCTCTGC |
| Exon12 | COL2A1_E12SF | GTGGAGTCTGAAGGAGGTGA |
| Exon13 | COL2A1_E13-14F | GTCACTTCTGAGATGAAACGCC |
| Exon14 | COL2A1_E13-14R | TCAGCTCGCACTGACACAAAC |
| Exon15 | COL2A1_E15R2 | TTACAAGCACACCTTGCTCC |
| Exon16 | COL2A1_E16SF | ACCATCAGGAGGTCCTTGTT |
| Exon17 | COL2A1_E17F | TCGCACAGACACCAAAACTGCA |
| Exon18 | COL2A1_E18F | CTTCTCACCACAGATGTAGTCA |
| Exon19 | COL2A1_E19F | AACTGTGTGTGAACCGACATGTTC |
| Exon20 | COL2A1_E20-22F | CTTGAAACACATAGTGGGAATGTC |
| Exon21-22 | COL2A1_E20-22R | CTGTGCCTCATAGAACAGCAG |
| Exon23 | COL2A1_E23F | GTGAACAGTTGGATCTTTAG |
| Exon24-25 | COL2A1_E24SR | TCTGAAGCCAAGGGCAACAG |
| Exon26 | COL2A1_E25-26SF | AAGGTGAGTGGGAGAAGAGG |
| Exon27 | COL2A1_E27-28F | TAGATGCTGAGCATGTGTGG |
| Exon28 | COL2A1_E27-28R | GATCAACACTCAATACTGAGG |
| Exon29 | COL2A1_E29-30F | AGTACCTTTGCCCAATCCTA |
| Exon30 | COL2A1_E29-30R | CGAAGTGACAAGCGTTAGCA |
| Exon31 | COL2A1_E31-33F | CTGGACAGCAGCAGGCACTATC |
| Exon32 | COL2A1_E31-33R | GACTCCAGGCTACCACGAAG |
| Exon33 | COL2A1_E33-34F | AATGCGGGCTGCCTCCTT |
| Exon34 | COL2A1_E34SR | CTTCATCACCAGGTGCCATA |
| Exon35 | COL2A1_E35SF | CACAGCAAATTCCTCTTGGG |
| Exon36 | COL2A1_E36-37F | GTCTGGGCAGTCTGCCACTG |
| Exon37 | COL2A1_E36-37R | GCAGGCAGAGGCTCTGTTAA |
| Exon38 | COL2A1_E38-39F | GCACGTCACTCCCATCATGT |
| Exon39 | COL2A1_E38-39R | CAGGAGATCAGCAGCTTGGT |
| Exon40 | COL2A1_E40-41F | ACCAAGCTGCTGATCTCCTG |
| Exon41 | COL2A1_E40-41R | TCAGTTAGCTACTCCTCCAG |
| Exon42 | COL2A1_E42SF | AGAGGAAACTGCTGTCACTG |
| Exon43 | COL2A1_E43SF | CTTCTGAGCTCACAGAGCAT |
| Exon44 | COL2A1_E44-45F | CAAGGCTTCTACCTCCCTCA |
| Exon45 | COL2A1_E44-45R | TCTAGGCTGAGATGAGACTTGTTC |
| Exon46 | COL2A1_E46-47F | GTTGGAACAAGTCTCATCTCA |
| Exon47 | COL2A1_E46-47R | GACAGACACCGATTGAGTCAGGTCA |
| Exon48 | COL2A1_E48-49F | TCCGCAGTCTCTGGACTAAG |
| Exon49 | COL2A1_E49SF | TGGGATCACCCTAAGCAGTT |
| Exon50 | COL2A1_E50-51F | ACTGAGCATGTGAAGAACTG |
| Exon51 | COL2A1_E50-51R | AACCCTCTGGCGGAAACTTC |
| Exon52 | COL2A1_E52-53F | CTTAGGGCTGGACTTAGCTC |
| Exon53 | COL2A1_E52-53R | TCTCTCACCTGTCACTCAGC |
| Exon54 | COL2A1_E54F | TGGAGAGGCCTTTGGCAAGC |
連絡先
お問い合わせは、
山田（taka0197@med.hokudai.ac.jp）宛