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COL2A1

COL2A1 PCR Primer
TargetPrimer nameSense primer (5'->3')Anti-sense primer (5'->3')Product size [bp]Annealing temperature[oC]
Exon1COL2A1_E1F/E1RAGAAGACGCAGAGCGCTGCTACAGAGTCTTGATTGGCAG437*
Exon2COL2A1_E2F/E2RGCCTACATTCTTCAGCATTTCTAGTGTCTCAGGCTCATTCACT521*
Exon3COL2A1_E3F2/E3RTCAGTGGAGTCACAGGAAGCTGCTGGATTAACATAGCATTGC242*
Exon4-5COL2A1_E4-5F/E4-5RGCAATGCTATGTTAATCCAGTCACCTTTGTCACCACGATC478*
Exon6-7 COL2A1_E6-7F/E6-7RGCTCTCTCTCACAGATTGTAGTAAGTGCAAGCAGCAATT548*
Exon8COL2A1_E8F2/E8R2CCAGGCATCAGTGGTAATTCTTCTTAGTCACTCCAGCGCA442*
Exon9-10COL2A1_E9-10F/E9-10RGCAGACAGCAGCTACTGTTCTACAGCAGCTGCGTCCTAGT624*
Exon11COL2A1_E11F/E11RAATCCCACTATGATCTCTGCCAGTCTTGCTCCTCAAGATAC329*
Exon12COL2A1_E12F/E12RCAGGATGTCTACAAAGGATGCCAAAGTGGAGGTGTTCAGAG816*
Exon13COL2A1_E13-14F/E13-14RGTCACTTCTGAGATGAAACGCCTCAGCTCGCACTGACACAAAC745*
Exon15COL2A1_E15F2/E15R2TCCTCAGAACAGCCTTGGCTTTACAAGCACACCTTGCTCC537*
Exon16COL2A1_E16SF/E16R2ACCATCAGGAGGTCCTTGTTGGGATTACCTGATAATCTGG445*
Exon17COL2A1_E17F/E17RTCGCACAGACACCAAAACTGCAATACACCCTCGAGACTGCCTTG291*
Exon18COL2A1_E18F/E18RCTTCTCACCACAGATGTAGTCAGTTGTTGAGGGAGCAATGAGCAAG368*
Exon19 COL2A1_E19F/E19RAACTGTGTGTGAACCGACATGTTCCAGAATGAAGGTTTGGTGGTTG365*
Exon20-22COL2A1_E20-22F/E19-21RCTTGAAACACATAGTGGGAATGTCGACTCCAGAGATGTCAGTGGAAC415*
Exon20-22COL2A1_E20-22F/E20-22RCTTGAAACACATAGTGGGAATGTC CTGTGCCTCATAGAACAGCAG 666*
Exon23COL2A1_E23F/E23RGTGAACAGTTGGATCTTTAGCAGAACACGGACCACAAGGACT380*
Exon24-26COL2A1_E24-26F/E24-26RCAGAAGTTAACCTCTGAGAATCCATCTCTCTTTTCCCTTGCTTC649*
Exon27-28COL2A1_E27-28F/E27-28RTAGATGCTGAGCATGTGTGGGATCAACACTCAATACTGAGG787*
Exon29-30COL2A1_E29-30F/E29-30RAGTACCTTTGCCCAATCCTACGAAGTGACAAGCGTTAGCA774*
Exon31-33COL2A1_E31-33F/E31-33RCTGGACAGCAGCAGGCACTATCGACTCCAGGCTACCACGAAG828*
Exon34COL2A1_E34-35F/E34-35RCGGGCTCAGGAGGAATGAAGGATCCTAATGCCCAGCAGTC711*
Exon35COL2A1_E35F2/E35R2GTGACATCCTTTCCCCTGTTAAGGTGTGGAGAGGAAAGGA599*
Exon36-37COL2A1_E36-37F/E36-37RGTCTGGGCAGTCTGCCACTGGCAGGCAGAGGCTCTGTTAA928*
Exon38-39COL2A1_E38-39F/E38-39RGCACGTCACTCCCATCATGTCAGGAGATCAGCAGCTTGGT840*
Exon40-41COL2A1_E40-41F/E40-41RACCAAGCTGCTGATCTCCTGTCAGTTAGCTACTCCTCCAG976*
Exon42-43COL2A1_E42-43F/E42-43RTGTCTCACATGGTGAGAAGGTTGCTTCAGGAGAGGGCAGACAAG761*
Exon44-45COL2A1_E44-45F/E44-45RCAAGGCTTCTACCTCCCTCATCTAGGCTGAGATGAGACTTGTTC751*
Exon46-47COL2A1_E46-47F/E46-47RGTTGGAACAAGTCTCATCTCAGACAGACACCGATTGAGTCAGGTCA591 *
Exon48-49COL2A1_E48-49F/E48-49RTCCGCAGTCTCTGGACTAAGTCTGTCTGACAGCGAGAGGCT960*
Exon50-51COL2A1_E50-51F/E50-51RACTGAGCATGTGAAGAACTGAACCCTCTGGCGGAAACTTC1007*
Exon52-53COL2A1_E52-53F/E52-53RCTTAGGGCTGGACTTAGCTCTCTCTCACCTGTCACTCAGC1131*
Exon54COL2A1_E54F/E54RTGGAGAGGCCTTTGGCAAGCTCAGGTCAGCCATTCAGTGC 417*

*Unless otherwise noted, Roche Diagnostics GmbH Expand high Fidelity PCR System used denaturation at 94 oC for 30 sec annealing at 60 oC for 1 min extension at 72 oC for 1 min × 5 Cycles, and denaturatiion at 95 oC for 30 sec annealing at 55 oC for 1 min extention at 72 oC for 1 min × 35 Cycles.

COL2A1 Sequence Primer
TargetPrimer nameSequence
Exon1COL2A1_E1FAGAAGACGCAGAGCGCTGCT
Exon2COL2A1_E2FGCCTACATTCTTCAGCATT
Exon3COL2A1_E3RCTGGATTAACATAGCATTGC
Exon4-5COL2A1_E4-5FGCAATGCTATGTTAATCCAG
Exon6-7COL2A1_E6-7RGTAAGTGCAAGCAGCAATT
Exon8COL2A1_E8F2CCAGGCATCAGTGGTAATTC
Exon9-10COL2A1_E9-10RACAGCAGCTGCGTCCTAGT
Exon11COL2A1_E11FAATCCCACTATGATCTCTGC
Exon12COL2A1_E12SFGTGGAGTCTGAAGGAGGTGA
Exon13COL2A1_E13-14FGTCACTTCTGAGATGAAACGCC
Exon14COL2A1_E13-14RTCAGCTCGCACTGACACAAAC
Exon15COL2A1_E15R2TTACAAGCACACCTTGCTCC
Exon16COL2A1_E16SFACCATCAGGAGGTCCTTGTT
Exon17COL2A1_E17FTCGCACAGACACCAAAACTGCA
Exon18COL2A1_E18FCTTCTCACCACAGATGTAGTCA
Exon19COL2A1_E19F AACTGTGTGTGAACCGACATGTTC
Exon20COL2A1_E20-22FCTTGAAACACATAGTGGGAATGTC
Exon21-22COL2A1_E20-22RCTGTGCCTCATAGAACAGCAG
Exon23COL2A1_E23F GTGAACAGTTGGATCTTTAG
Exon24-25COL2A1_E24SRTCTGAAGCCAAGGGCAACAG
Exon26COL2A1_E25-26SFAAGGTGAGTGGGAGAAGAGG
Exon27COL2A1_E27-28FTAGATGCTGAGCATGTGTGG
Exon28COL2A1_E27-28RGATCAACACTCAATACTGAGG
Exon29COL2A1_E29-30FAGTACCTTTGCCCAATCCTA
Exon30COL2A1_E29-30RCGAAGTGACAAGCGTTAGCA
Exon31COL2A1_E31-33FCTGGACAGCAGCAGGCACTATC
Exon32COL2A1_E31-33RGACTCCAGGCTACCACGAAG
Exon33COL2A1_E33-34FAATGCGGGCTGCCTCCTT
Exon34COL2A1_E34SRCTTCATCACCAGGTGCCATA
Exon35COL2A1_E35SFCACAGCAAATTCCTCTTGGG
Exon36COL2A1_E36-37FGTCTGGGCAGTCTGCCACTG
Exon37COL2A1_E36-37RGCAGGCAGAGGCTCTGTTAA
Exon38COL2A1_E38-39FGCACGTCACTCCCATCATGT
Exon39COL2A1_E38-39RCAGGAGATCAGCAGCTTGGT
Exon40COL2A1_E40-41FACCAAGCTGCTGATCTCCTG
Exon41COL2A1_E40-41RTCAGTTAGCTACTCCTCCAG
Exon42COL2A1_E42SFAGAGGAAACTGCTGTCACTG
Exon43COL2A1_E43SFCTTCTGAGCTCACAGAGCAT
Exon44COL2A1_E44-45FCAAGGCTTCTACCTCCCTCA
Exon45COL2A1_E44-45RTCTAGGCTGAGATGAGACTTGTTC
Exon46COL2A1_E46-47FGTTGGAACAAGTCTCATCTCA
Exon47COL2A1_E46-47RGACAGACACCGATTGAGTCAGGTCA
Exon48COL2A1_E48-49FTCCGCAGTCTCTGGACTAAG
Exon49COL2A1_E49SFTGGGATCACCCTAAGCAGTT
Exon50COL2A1_E50-51FACTGAGCATGTGAAGAACTG
Exon51COL2A1_E50-51RAACCCTCTGGCGGAAACTTC
Exon52COL2A1_E52-53FCTTAGGGCTGGACTTAGCTC
Exon53COL2A1_E52-53RTCTCTCACCTGTCACTCAGC
Exon54COL2A1_E54FTGGAGAGGCCTTTGGCAAGC
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連絡先

お問い合わせは、
遺伝子解析、DNA診断に関するもの:
山田(taka0197@med.hokudai.ac.jp)宛

診断/X線診断に関するもの:
西村 (gen-n@pc4.so-net.ne.jp)宛
治療に関するもの:
池川(sikegawa@ims.u-tokyo.ac.jp)宛

でお願いします。なお、臨床像のファイルが5MB以上になる場合は、CDかファイルを分割して送ってください。